HPLC Column, RP C12, 10um, 4.6mm ID x 250mm Length, 100A. Cogent Brand. 1 EA.
Additional Information:
Cogent RP C12 is a unique reversed-phase HPLC stationary phase based on an adamantane bonded ligand, a rigid diamond-like carbon structure that provides distinctive chromatographic selectivity compared to traditional alkyl phases. The combination of high-purity silica, a narrow particle size distribution, low metal content, complete double endcapping, and the rigid bonded phase design makes this column particularly effective for resolving complex mixtures, fast gradient separations, and applications requiring rapid equilibration between runs. The phase generally provides retention that falls between C8 and C18 columns, making it an excellent choice when C18 retention is excessive and C8 retention is insufficient.
68012-25P is an analytical HPLC column with dimensions of 4.6 mm ID x 250 mm length, packed with 10 µm RP C12 particles featuring a 100 Å pore size. The 250 mm column length provides maximum resolving power and peak capacity for challenging separations involving closely eluting compounds and complex sample matrices. The rigid adamantane bonded phase promotes rapid re-equilibration during gradient methods, while the 10 µm particle size supports higher flow rates and lower operating backpressure. Typical flow rates for this 4.6 mm ID column are approximately 1.0–2.0 mL/min depending on mobile phase composition and system requirements.
Compatible with reversed-phase mobile phases, including 100% aqueous conditions, this column maintains reproducible retention without loss of performance between runs. Typical applications include pharmaceutical analysis, quality control testing, environmental analysis, method development, high-throughput screening, and complex mixture separations. The extended column length is particularly advantageous when maximum resolution is required for difficult separations. This column is also frequently used for preparative method scouting before scaling methods to larger diameter purification columns.
Key Benefits
Unique adamantane-based RP C12 stationary phase
Fully double endcapped chemistry
High-purity silica with low metal content
Narrow particle size distribution
Compatible with 100% aqueous mobile phases
Rapid equilibration between runs
Excellent for complex mixture separations
Retention between C8 and C18 phases
10 µm particles for lower operating backpressure
Maximum resolving power with 250 mm length
Typical Applications
Analytical HPLC
High-throughput screening (HTS)
Method development
Fast gradient analysis
Pharmaceutical analysis
Quality control testing
Environmental analysis
Complex mixture separations
Preparative method scouting
Routine laboratory testing
Specifications
Product Type: HPLC Column
Stationary Phase: RP C12
Chromatography Mode: Reversed Phase
Bonded Phase: Adamantane-Based C12 Ligand
Endcapping: Fully Double Endcapped
Particle Size: 10 µm
Pore Size: 100 Å
Column Dimensions: 4.6 mm ID x 250 mm
Length: 250 mm
Typical Flow Rate: 1.0–2.0 mL/min
Brand: Cogent
Additional Resources
Important Notes
Compatible with 100% aqueous mobile phases
Excellent choice when C18 retention is too great and C8 retention is insufficient
Rapid re-equilibration supports high-speed gradient methods
Frequently selected for complex mixture separations
Extended column length can maximize resolution for difficult separations
Often used to scout methods prior to preparative purification scale-up
A Cogent Column Filter is included and recommended to help protect the column and extend service life